The 20 naturally occurring amino acids can be of either one of two optical isomers, L or D amino acids. These are each others mirror images. The L-form, which is most commonly found in nature, is assumed in the standard notation. The incorporation of D amino acids can be made, as in the W-peptide, and is in most cases straight forward.
A point of consideration is the quality of the reagents used. When producing high quality peptides, this is always important, but for peptides containing D-amino acids, this becomes pivotal. Since the L- and D form of the amino acids have identical physiochemical properties, it is not possible to purify the material by HPLC in order to separate the two. Thus, high quality reagents are required in order to obtain a high purity end product.
Innovagen has serviced the research community since 1992. We believe that quality always pays off and we will go that extra mile for the benefit of your project. Over the years we have been providing a number of D-amino acid containing peptides.
Examples of references of customers using our custom peptide synthesis service, where D-amino acids have been incorporated;
A. de Paulis, N. Prevete, I. Fiorentino, A.F. Walls, M. Curto, A. Petraroli, V. Castaldo, P. Ceppa, R. Fiocca, G. Marone. 2004. Basophils Infiltrate Human Gastric Mucosa at Sites of Helicobacter pylori Infection, and Exhibit Chemotaxis in Response to H. pylori-derived Peptide Hp(2-20).
Journal of Immunology, 2004, 172: 7734-7743.
A. A. Strömstedt, M. Pasupuleti, A. Schmidtchen & M. Malmsten
Evaluation of Strategies for Improving Proteolytic Resistance of Antimicrobial Peptides by Using Variants of EFK17, an Internal Segment of LL-37.
Antimicrobial Agents and Chemotherapy, 2009, p. 593–602 Vol. 53, No. 2
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