There are no reliable algorithms that we are aware of, that will accurately predict stability or solubility of a given peptide. That may change in the future, but today these things would have to be determined by experiment. In our experience, there are however general guidelines that we are happy to share with you.
For long term storage, peptides should be stored lyophilised, airtight, desiccated and at -20°C or colder. Stored in this fashion, peptides in general tend to be stable for several years. Compared to any scenario where the peptide is re-suspended, storing dry material is likely to be the better choice as far as stability goes. Of course, one should avoid freeze/thaw cycles and thus aliquoting is a good idea. However, since weighing out lyophilised aliquots is a hassle at best and downright impossible at times, it is perhaps not something that you would look forward to doing yourself and might be better left to the laboratory that produces the material. As a result, when you turn to us for your peptide requirements, we offer to deliver the material in aliquot sizes that you specify. It’s a small cost for you and money well spent.
Most peptides will dissolve in pure water, and any buffer components you’d like to have should be added to the peptide solution. If you want to make an educated guess about the solubility, you can look for charged amino acids in the sequence. These are Arg (R), Lys (K), Asp (D) and Glu (E). The more charges you have the more likely it is that the peptide will dissolve in pure water.
Enter your sequence here and see what our peptide property calculator has to report.
When the peptide doesn’t dissolve in pure water, it is sometimes sufficient to add a base or acid to shift the pH away from the iso-electric point of the peptide. The beta amyloid 1-40 is an example of such a peptide, where elevating the pH will make it go into solution more easily. Other tricks like gentle heating or sonication can be used. When your application will allow for it, using an organic solvent such as DMSO is a popular option. Often the peptide can be re-suspended in a small volume of solvent and then be diluted further to working concentration.
Since 1992 Innovagen has been a provider of research materials to scientists in most, if not all, major labs world wide. Our knowledge comes from the experience gained over 20 or so years from the thousands of peptide projects that we have been involved in. You too will benefit from our dedication to the production of high quality peptides and our excellent customer service when you turn to us for your peptide requirements.
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