Förster (or Fluorescence) Resonance Energy Transfer, FRET, is a non-radiative energy transfer between two chromophores, which are not necessarily fluorescent. The interaction is highly distance dependent and suitable for researching phenomena that are manifested in changes in close proximity. Depending on the detection technique employed, a donor-acceptor pair can be two different chromophores or the same, either be fluorescent or not. Using a fluorescent donor and a non-fluorescent acceptor has the advantage of eliminating background fluorescence due to direct acceptor excitation.
Due to the fact that peptides are synthesized from C-terminus to N-terminus, employing a strategy where an acetylation cycle is run for each amino acid coupling step, only full length peptide will carry the N-terminal chromophore. Conversely, all peptide molecules will have the C-terminal label. In this case, it is advantageous to have the acceptor as the C-terminal chromophore.
Innovagen has serviced the research community since 1992. Over the years we have been providing numerous synthetic peptides for all sorts of applications, including FRET pairs. From this experience, we know how just choosing the right synthesis strategy can make a world of difference for the final product. But our commitment goes beyond this as we believe that quality always pays off and we will go that extra mile for the benefit of your project. Please take a few moments to read about our view on production and quality control.
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