Traditionally used and with an abundance of secondary antibodies, rat-antibodies are suitable for most projects. What is more, for anti-peptide antibody projects, where we have designed and produced the peptide, we will guarantee that the resulting antiserum will contain antibodies that target the peptide.
Of course, you can fully customise your project, but for your convenience we have outlined a typical project.
An immunogen is required for the antibody production. This can be a protein or other molecule that you provide, or it can be produced here. In the latter case, either the entire protein can be obtained using a recombinant technique, or a peptide is designed and synthesized. Using peptides as antigens is the most frequently used strategy. It should be noted that the more effort that is put into this step, the better the odds for the successful outcome of your project. Other labs may choose to ignore this fact, but the production of high quality peptides for antibody production may very well be pivotal for the success of your project.
If you will be supplying the immunogen, there are a few things to keep in mind;
One or two rats are immunised using our standard protocol. For each rat pre-immune serum is collected prior to immunisations, and two test bleeds are made over the course of the immunisation schedule. The protocol ends in a terminal bleed yielding approximately 3 ml serum.
The crude rat antiserum often works very well without any purification. If you wish, we can purify serum and collect either the total IgG, or the fraction of antigen specific antibodies.
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