Rabbit is the host of choice for producing polyclonal antibodies, and for good reason. As rabbits have been routinely used for a long time, the entire apparatus from rabbit breeds, facilities, to immunisation protocols and secondary antibodies, have been thoroughly tested and evaluated for best performance. There are merits to using chicken, but unless antibody amounts or phylogenetic distance between the host and the species of the target protein are of importance, rabbits remain the most commonly asked for host.
The concentration of total IgG in rabbit antiserum is 5-10 mg/ml, and 1-10% of this are the sought after antigen specific antibodies. A typical bleed results in 20 ml serum, and thus, expected to contain 1-20 mg of specific antibody. The typical yield when purifying a 20 ml serum sample is 1-5 mg.
Innovagen was founded in 1992. Over the years we have seen thousands of polyclonal rabbit antibody projects, against various types of antigens. When turning to us for your antibody requirements, you will benefit from our experience and commitment to quality.
Examples of references of customers using our custom rabbit antibody services include;
H. Han, D. Xu, C. Liu, H-E. Claesson, M. Björkholm, J. Sjöberg
Interleukin-4-Mediated 15-Lipoxygenase-1 Trans-Activation Requires UTX Recruitment and H3K27me3 Demethylation at the Promoter in A549 Cells
PLOS, January 20, 2014 doi: 10.1371/journal.pone.0085085
R. Bahtz, J. Seidler, M. Arnold, U. Haselmann-Weiss, C. Antony, W. D. Lehmann and I. Hoffmann
GCP6 is a substrate of Plk4 and required for centriole duplication
Journal of Cell Science, February 2, 2012 doi: 10.1242/jcs.093930
H. H. Qi, P. P. Ongusaha, J. Myllyharju, D. Cheng, O. Pakkanen, Y. Shi, S. W. Lee, J. Peng & Y. Shi.
Prolyl 4-hydroxylation regulates Argonaute 2 stability.
Nature, 18 September, 2008, 455, 421-424
M. W. Hornef, T. Frisan, A. Vandewalle, S. Normark, and A. Richter-Dahlfors.
Toll-like Receptor 4 Resides in the Golgi Apparatus and Colocalizes with Internalized Lipopolysaccharide in Intestinal Epithelial Cells.
Journal of Experimental Medicine, February 25, 2002, vol. 195 no. 5 559-570.
L. Camper, U. Hellman, E. Lundgren-Åkerlund
Isolation, Cloning, and Sequence Analysis of the Integrin Subunit α10, a β1-associated Collagen Binding Integrin Expressed on Chondrocytes.
The Journal of Biological Chemistry, August 7, 1998, vol 273, 20383-20389.
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