After successful immunisations, and your evaluation of the mouse serum, we offer to perform cell fusions for the purpose of obtaining a stable monoclonal cell line.
Fusions of cells from one or two spleens with myeloma cells are made. Following this, the screening for the selection of clones is typically done only with the antigen used for immunisations. It is however quite possible to extend the screening to entail several substrates, allowing for a more discriminating selection. For example, when an antigenic synthetic peptide has been used for immunisations, one might want to include the target protein as a second analyte.
Antibody containing supernatant from the positive mother clones are made available to you for testing. Once one or more clones have been selected these are subjected to subcloning and another screening round. The objective is to obtain isolated cells, and thus the monoclonal cell line. You will receive supernatant from positive clones for testing. Selected clones are then sent to you or used in additional antibody services, most commonly antibody production.
Innovagen was founded in 1992. Since then we have been providing the scientific community with high quality research antibodies and their related services. Our experience and dedicated customer service will ensure that your best interests are looked after when you turn to us with your antibody project.
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