Custom service: phospho-specific antibody

We provide a custom phospho-specific antibody production service. Typically such a project is a rabbit polyclonal anti-peptide antibody, which is what is outlined in the below. However, should your requirements differ from this they can be accommodated. Please contact us for a free consultation in order to determine your requirements as well as project feasibility and estimated cost.

The phospho-specific service package includes;

• Design and synthesis of phospho immunisation peptide (10 mg, high purity) and a non-phospho control peptide.
• Conjugation of the immunisation peptide to KLH
• Immunisation of two rabbits using a 12 week standard protocol.
• Collection of pre-immune serum and three immune serum samples.
• ELISA testing of first serum sample against the phospho and non-phospho peptides.
• Phospho specific affinity purification using a total of 20 ml of crude serum.
  The antibody is tested by ELISA to show reactivity with the phosphorylated peptide,
  no reactivity with the des-phospho peptide, and no reactivity with an unrelated phosphorylated sequence.

Additional services can be added to this package. Some examples include;

• Antibody aliquotation
• Antibody labelling, such as biotin or FITC.

The projected time line for your custom phospho-specific polyclonal rabbit antibody project is as follows;

Addtional information

• Phospho-specific antibodies
• Antibodies raised against synthetic peptides

Order phospho-specific antibodies

• Contact us for a free consultation
• Order custom phospho-specific antibody